LymphedemaV1, Main, Exploration, bibRecord, 009675

Lymphatic endothelial reprogramming of vascular endothelial cells by the Prox‐1 homeobox transcription factor

Identifieur interne : 009675 ( Main/Exploration ); précédent : 009674; suivant : 009676

Lymphatic endothelial reprogramming of vascular endothelial cells by the Prox‐1 homeobox transcription factor

Auteurs : Tatiana V. Petrova [États-Unis] ; Taija M Kinen [États-Unis] ; Tomi P. M Kel [États-Unis] ; Janna Saarela [États-Unis] ; Ismo Virtanen [États-Unis] ; Robert E. Ferrell [États-Unis] ; David N. Finegold [États-Unis] ; Dontscho Kerjaschki [Autriche] ; Seppo Yl Erttuala [Finlande] ; Kari Alitalo [États-Unis, Finlande]

Source :

The EMBO Journal [ 0261-4189 ] ; 2002-09-02.

RBID : ISTEX:40A29E8710124BFC26B3BB6B998B5DF45CDE55B9

Abstract

Lymphatic vessels are essential for fluid homeostasis, immune surveillance and fat adsorption, and also serve as a major route for tumor metastasis in many types of cancer. We found that isolated human primary lymphatic and blood vascular endothelial cells (LECs and BECs, respectively) show interesting differences in gene expression relevant for their distinct functions in vivo. Although these phenotypes are stable in vitro and in vivo, overexpression of the homeobox transcription factor Prox‐1 in the BECs was capable of inducing LEC‐specific gene transcription in the BECs, and, surprisingly, Prox‐1 suppressed the expression of ∼40% of the BEC‐specific genes. Prox‐1 did not have global effects on the expression of LEC‐specific genes in other cell types, except that it up‐regulated cyclin E1 and E2 mRNAs and activated the cyclin e promoter in various cell types. These data suggest that Prox‐1 acts as a cell proliferation inducer and a fate determination factor for the LECs. Furthermore, the data provide insights into the phenotypic diversity of endothelial cells and into the possibility of transcriptional reprogramming of differentiated endothelial cells.

Url:

DOI: 10.1093/emboj/cdf470

Affiliations:

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lymphatic endothelial reprogramming of vascular endothelial cells by the Prox‐1 homeobox transcription factor</title>
<author><name sortKey="Petrova, Tatiana V" sort="Petrova, Tatiana V" uniqKey="Petrova T" first="Tatiana V." last="Petrova">Tatiana V. Petrova</name>
</author>
<author><name sortKey="M Kinen, Taija" sort="M Kinen, Taija" uniqKey="M Kinen T" first="Taija" last="M Kinen">Taija M Kinen</name>
</author>
<author><name sortKey="M Kel, Tomi P" sort="M Kel, Tomi P" uniqKey="M Kel T" first="Tomi P." last="M Kel">Tomi P. M Kel</name>
</author>
<author><name sortKey="Saarela, Janna" sort="Saarela, Janna" uniqKey="Saarela J" first="Janna" last="Saarela">Janna Saarela</name>
</author>
<author><name sortKey="Virtanen, Ismo" sort="Virtanen, Ismo" uniqKey="Virtanen I" first="Ismo" last="Virtanen">Ismo Virtanen</name>
</author>
<author><name sortKey="Ferrell, Robert E" sort="Ferrell, Robert E" uniqKey="Ferrell R" first="Robert E." last="Ferrell">Robert E. Ferrell</name>
</author>
<author><name sortKey="Finegold, David N" sort="Finegold, David N" uniqKey="Finegold D" first="David N." last="Finegold">David N. Finegold</name>
</author>
<author><name sortKey="Kerjaschki, Dontscho" sort="Kerjaschki, Dontscho" uniqKey="Kerjaschki D" first="Dontscho" last="Kerjaschki">Dontscho Kerjaschki</name>
</author>
<author><name sortKey="Yl Erttuala, Seppo" sort="Yl Erttuala, Seppo" uniqKey="Yl Erttuala S" first="Seppo" last="Yl Erttuala">Seppo Yl Erttuala</name>
</author>
<author><name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name>
<affiliation><country>Finlande</country>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:40A29E8710124BFC26B3BB6B998B5DF45CDE55B9</idno>
<date when="2002" year="2002">2002</date>
<idno type="doi">10.1093/emboj/cdf470</idno>
<idno type="url">https://api.istex.fr/document/40A29E8710124BFC26B3BB6B998B5DF45CDE55B9/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001D63</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001D63</idno>
<idno type="wicri:Area/Istex/Curation">001D63</idno>
<idno type="wicri:Area/Istex/Checkpoint">002618</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002618</idno>
<idno type="wicri:doubleKey">0261-4189:2002:Petrova T:lymphatic:endothelial:reprogramming</idno>
<idno type="wicri:source">PMC</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC125413</idno>
<idno type="RBID">PMC:125413</idno>
<idno type="wicri:Area/Pmc/Corpus">001753</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001753</idno>
<idno type="wicri:Area/Pmc/Curation">001752</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001752</idno>
<idno type="wicri:Area/Pmc/Checkpoint">003F52</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">003F52</idno>
<idno type="wicri:Area/Ncbi/Merge">000A86</idno>
<idno type="wicri:Area/Ncbi/Curation">000A86</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000A86</idno>
<idno type="wicri:doubleKey">0261-4189:2002:Petrova T:lymphatic:endothelial:reprogramming</idno>
<idno type="wicri:Area/Main/Merge">009A56</idno>
<idno type="wicri:Area/Main/Curation">009675</idno>
<idno type="wicri:Area/Main/Exploration">009675</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">Lymphatic endothelial reprogramming of vascular endothelial cells by the Prox‐1 homeobox transcription factor</title>
<author><name sortKey="Petrova, Tatiana V" sort="Petrova, Tatiana V" uniqKey="Petrova T" first="Tatiana V." last="Petrova">Tatiana V. Petrova</name>
<affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Molecular/Cancer Biology Laboratory and Ludwig Institute for Cancer Research, Haartman Institute and Helsinki University Central Hospital, 00014, Helsinki</wicri:regionArea>
<wicri:noRegion>Helsinki</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="M Kinen, Taija" sort="M Kinen, Taija" uniqKey="M Kinen T" first="Taija" last="M Kinen">Taija M Kinen</name>
<affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Molecular/Cancer Biology Laboratory and Ludwig Institute for Cancer Research, Haartman Institute and Helsinki University Central Hospital, 00014, Helsinki</wicri:regionArea>
<wicri:noRegion>Helsinki</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="M Kel, Tomi P" sort="M Kel, Tomi P" uniqKey="M Kel T" first="Tomi P." last="M Kel">Tomi P. M Kel</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cell Cycle Laboratory, Biomedicum Helsinki, University of Helsinki, 00014, Helsinki</wicri:regionArea>
<orgName type="university">Université d'Helsinki</orgName>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Saarela, Janna" sort="Saarela, Janna" uniqKey="Saarela J" first="Janna" last="Saarela">Janna Saarela</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>National Public Health Institute, Biomedicum Helsinki, University of Helsinki, 00014, Helsinki</wicri:regionArea>
<orgName type="university">Université d'Helsinki</orgName>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Virtanen, Ismo" sort="Virtanen, Ismo" uniqKey="Virtanen I" first="Ismo" last="Virtanen">Ismo Virtanen</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Anatomy, Biomedicum Helsinki, University of Helsinki, 00014, Helsinki</wicri:regionArea>
<orgName type="university">Université d'Helsinki</orgName>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ferrell, Robert E" sort="Ferrell, Robert E" uniqKey="Ferrell R" first="Robert E." last="Ferrell">Robert E. Ferrell</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Human Genetics, University of Pittsburgh, PA, Pittsburgh</wicri:regionArea>
<orgName type="university">Université de Pittsburgh</orgName>
<placeName><settlement type="city">Pittsburgh</settlement>
<region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Finegold, David N" sort="Finegold, David N" uniqKey="Finegold D" first="David N." last="Finegold">David N. Finegold</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Human Genetics, University of Pittsburgh, Pittsburgh, PA</wicri:regionArea>
<placeName><region type="state">Pennsylvanie</region>
<settlement type="city">Pittsburgh</settlement>
</placeName>
<orgName type="university">Université de Pittsburgh</orgName>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pediatrics, University of Pittsburgh, PA, Pittsburgh</wicri:regionArea>
<orgName type="university">Université de Pittsburgh</orgName>
<placeName><settlement type="city">Pittsburgh</settlement>
<region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kerjaschki, Dontscho" sort="Kerjaschki, Dontscho" uniqKey="Kerjaschki D" first="Dontscho" last="Kerjaschki">Dontscho Kerjaschki</name>
<affiliation wicri:level="3"><country xml:lang="fr">Autriche</country>
<wicri:regionArea>Department of Pathology, University of Vienna Medical School, 1090, Vienna</wicri:regionArea>
<placeName><settlement type="city">Vienne (Autriche)</settlement>
<region nuts="2" type="province">Vienne (Autriche)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yl Erttuala, Seppo" sort="Yl Erttuala, Seppo" uniqKey="Yl Erttuala S" first="Seppo" last="Yl Erttuala">Seppo Yl Erttuala</name>
<affiliation wicri:level="1"><country xml:lang="fr">Finlande</country>
<wicri:regionArea>Department of Molecular Medicine, A.I. Virtanen Institute, University of Kuopio, 70211, Kuopio</wicri:regionArea>
<wicri:noRegion>Kuopio</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name>
<affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Molecular/Cancer Biology Laboratory and Ludwig Institute for Cancer Research, Haartman Institute and Helsinki University Central Hospital, 00014, Helsinki</wicri:regionArea>
<wicri:noRegion>Helsinki</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Finlande</country>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">The EMBO Journal</title>
<title level="j" type="alt">THE EMBO JOURNAL</title>
<idno type="ISSN">0261-4189</idno>
<idno type="eISSN">1460-2075</idno>
<imprint><biblScope unit="vol">21</biblScope>
<biblScope unit="issue">17</biblScope>
<biblScope unit="page" from="4593">4593</biblScope>
<biblScope unit="page" to="4599">4599</biblScope>
<biblScope unit="page-count">7</biblScope>
<publisher>John Wiley & Sons, Ltd</publisher>
<pubPlace>Chichester, UK</pubPlace>
<date type="published" when="2002-09-02">2002-09-02</date>
</imprint>
<idno type="ISSN">0261-4189</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0261-4189</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">Lymphatic vessels are essential for fluid homeostasis, immune surveillance and fat adsorption, and also serve as a major route for tumor metastasis in many types of cancer. We found that isolated human primary lymphatic and blood vascular endothelial cells (LECs and BECs, respectively) show interesting differences in gene expression relevant for their distinct functions in vivo. Although these phenotypes are stable in vitro and in vivo, overexpression of the homeobox transcription factor Prox‐1 in the BECs was capable of inducing LEC‐specific gene transcription in the BECs, and, surprisingly, Prox‐1 suppressed the expression of ∼40% of the BEC‐specific genes. Prox‐1 did not have global effects on the expression of LEC‐specific genes in other cell types, except that it up‐regulated cyclin E1 and E2 mRNAs and activated the cyclin e promoter in various cell types. These data suggest that Prox‐1 acts as a cell proliferation inducer and a fate determination factor for the LECs. Furthermore, the data provide insights into the phenotypic diversity of endothelial cells and into the possibility of transcriptional reprogramming of differentiated endothelial cells.</div>
</front>
</TEI>
<affiliations><list><country><li>Autriche</li>
<li>Finlande</li>
<li>États-Unis</li>
</country>
<region><li>Pennsylvanie</li>
<li>Uusimaa</li>
<li>Vienne (Autriche)</li>
</region>
<settlement><li>Helsinki</li>
<li>Pittsburgh</li>
<li>Vienne (Autriche)</li>
</settlement>
<orgName><li>Université d'Helsinki</li>
<li>Université de Pittsburgh</li>
</orgName>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Petrova, Tatiana V" sort="Petrova, Tatiana V" uniqKey="Petrova T" first="Tatiana V." last="Petrova">Tatiana V. Petrova</name>
</noRegion>
<name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name>
<name sortKey="Ferrell, Robert E" sort="Ferrell, Robert E" uniqKey="Ferrell R" first="Robert E." last="Ferrell">Robert E. Ferrell</name>
<name sortKey="Finegold, David N" sort="Finegold, David N" uniqKey="Finegold D" first="David N." last="Finegold">David N. Finegold</name>
<name sortKey="Finegold, David N" sort="Finegold, David N" uniqKey="Finegold D" first="David N." last="Finegold">David N. Finegold</name>
<name sortKey="M Kel, Tomi P" sort="M Kel, Tomi P" uniqKey="M Kel T" first="Tomi P." last="M Kel">Tomi P. M Kel</name>
<name sortKey="M Kinen, Taija" sort="M Kinen, Taija" uniqKey="M Kinen T" first="Taija" last="M Kinen">Taija M Kinen</name>
<name sortKey="Saarela, Janna" sort="Saarela, Janna" uniqKey="Saarela J" first="Janna" last="Saarela">Janna Saarela</name>
<name sortKey="Virtanen, Ismo" sort="Virtanen, Ismo" uniqKey="Virtanen I" first="Ismo" last="Virtanen">Ismo Virtanen</name>
</country>
<country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Kerjaschki, Dontscho" sort="Kerjaschki, Dontscho" uniqKey="Kerjaschki D" first="Dontscho" last="Kerjaschki">Dontscho Kerjaschki</name>
</region>
</country>
<country name="Finlande"><noRegion><name sortKey="Yl Erttuala, Seppo" sort="Yl Erttuala, Seppo" uniqKey="Yl Erttuala S" first="Seppo" last="Yl Erttuala">Seppo Yl Erttuala</name>
</noRegion>
<name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 009675 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 009675 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    LymphedemaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:40A29E8710124BFC26B3BB6B998B5DF45CDE55B9
   |texte=   Lymphatic endothelial reprogramming of vascular endothelial cells by the Prox‐1 homeobox transcription factor
}}

This area was generated with Dilib version V0.6.31.
Data generation: Sat Nov 4 17:40:35 2017. Site generation: Tue Feb 13 16:42:16 2024

Serveur d'exploration sur le lymphœdème

Lymphatic endothelial reprogramming of vascular endothelial cells by the Prox‐1 homeobox transcription factor

Lymphatic endothelial reprogramming of vascular endothelial cells by the Prox‐1 homeobox transcription factor

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

	Serveur d'exploration sur le lymphœdème
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.